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| Registros recuperados: 57 | |
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| Sauvage, Christopher; Pepin, Jean-francois; Lapegue, Sylvie; Boudry, Pierre; Renault, Tristan. |
| Ostreid herpes virus 1 (OsHV-1) infections, notably reported in Europe and the USA, are closely associated with significant mortalities of the Pacific oyster, Crassostrea gigas, especially during its early stages of life. In summer 2006, we monitored mortality by strict daily verification of three full-sib families of oysters reared under common conditions. We quantified OsHV-1 using real-time PCR in dead and living individuals during and after a mortality event. Mortality events were severe and brief, but significantly different between tested families (cumulative mortality ranging from 1.2 to 49%). Real-time PCR assays revealed different viral DNA loads in dead individuals from different families (P < 0.001). Moreover, the mean level of infection... |
| Tipo: Text |
Palavras-chave: Viral DNA quantification; Real time PCR; OsHV 1; Mortality; Crassostrea gigas. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6265.pdf |
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| Polsenaere, Pierre; Soletchnik, Patrick; Le Moine, Olivier; Gohin, Francis; Robert, Stephane; Pepin, Jean-francois; Stanisiere, Jean-yves; Dumas, Franck; Bechemin, Christian; Goulletquer, Philippe. |
| In the context of global change, increasing mariculture production has raised particular concerns regarding its environmental impact and sustainability. Molluscs and particularly blue mussel account for a significant part of this total production. Although blue mussels are considered as pretty resilient to environmental disturbances, we report in this study an unprecedented mussel mortality event that occurred during the winter 2014 in the Breton Sound. 9000 metric tonnes of mussels were lost and mortality rates up to 100% were recorded at some farming areas. Through a coupling approach, the present work aimed to better understand the potential environmental drivers associated to those mortalities. Firstly we analysed long-term in situ and satellite data... |
| Tipo: Text |
Palavras-chave: Mytilus edulis mortalities; Climate change; Breton sound; Long-term monitoring; Hydrodynamic modelling; Vibriosis. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00376/48708/49083.pdf |
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| Coeurdacier, Jean-luc; Laporte, Florent; Pepin, Jean-francois. |
| Cette présentation orale aborde les aspects non traités dans la publication relative aux essais de vaccination contre la Nodavirose par peptides synthétiques (https://w3.ifremer.fr/archimer/doc/00000/10600/7038.pdf) dont évaluation par différentes méthodes de la présence ou des traces d’un contact avec le Nodavirus qu’il soit vivant (culture de cellule), sous forme de fraction protéique (ELISA antigène et PCR) ou qu’il ait laissé des traces sériques (séropositivité par ELISA anticorps) ou sous forme de vacuoles encéphaliques (immunohistochimie). |
| Tipo: Text |
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| Ano: 1999 |
URL: http://archimer.ifremer.fr/doc/00157/26828/24943.pdf |
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| Pepin, Jean-francois; Bouget, Jean-francois; Chabirand, Jean-michel; Chasselin, Leo; Costes, Louis; Grizon, James; Lejolivet, Aurore; Le Noc, Sandrine; Palvadeau, Hubert; Schmitt, Anne; Seugnet, Jean-luc; Soletchnik, Patrick; Travers, Marie-agnes; Tourbiez, Delphine; Guesdon, Stephane. |
| Since 2014 in France, in Charente maritime and Vendée, farmed and stocks of blue mussels experienced strong mortality events. In this context, the « MORBLEU » research project (DPMA-Ifremer agreement) was initiated to explore potentially aggravating factors, associated or correlated with mortality of mussels. Here, the work is to study biotic factors in mussels environment, looking for describe diversity and evolution of microbial communities (water column) before, during and after mortality events using NGS technology (metabarcoding) for environmental DNA analysis. We present, sampling strategy used from 2015 to 2018, Material & Methods and some preliminary results. |
| Tipo: Text |
Palavras-chave: MORBLEU; Moules; Mortalité; ADN environnemental; EDNA; Metabarcoding; Communautés microbiennes. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00505/61664/65579.pdf |
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| Pepin, Jean-francois; Travers, Marie-agnes. |
| Durant l’hiver 2014 jusqu’à mai 2014, des mortalités exceptionnelles (50-100%) ont affectées la moule bleue, Mytilus edulis dans les Pertuis Charentais (principalement secteur du Pertuis Breton) et en Vendée. A la demande du Ministère (DPMA) une expertise environnementale a été demandée à l’Ifremer. L’expertise a permis de montrer que les conditions hydrologiques de l’hiver 2014 ne permettent pas de mettre en évidence une cause environnementale à ces mortalités exceptionnelles. Les analyses en pathologie réalisées ont révélées dans les moules la présence de bactéries appartenant au groupe bactérien Vibrio splendidus, dont la virulence a été confirmée expérimentalement. Dans ce contexte, la DPMA a sollicité l’Ifremer pour conduire en 20105 une étude... |
| Tipo: Text |
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| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00361/47174/47141.pdf |
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| Thomas, Gerard; Piquet, Jean-come; Ryckaert, Mireille; Masson, Daniel; Pepin, Jean-francois; Polsenaere, Pierre; Fillon, Alain. |
| Marine environment contamination can affect fishing and shellfish farming products (and shellfish in the first place), thus making them improper for human consumption, threatening public health and producer’s business. Analysis and health risk management are mandatory for consumer’s security and shellfish production survival. About microbial risk, monitoring shellfish production areas and several studies carried by LERPC laboratory give a good view of the microbial contamination impacting the Pertuis Charentais shellfish production area. On the other hand, State-Region Plan Contract (CPER) studies results from 2007 to 2013 are presented and discussed; additionally, the law-prescribed sanitary studies for professional on foot-fishing classification are... |
| Tipo: Text |
Palavras-chave: Microbiologie; E. coli; Norovirus; Étude sanitaire; Conchyliculture; Pêche à pied professionnelle; Pertuis Charentais; Microbiology; E.coli; Norovirus; Sanitary(health) studies; Shellfish farming; On foot]fishing; Pertuis Charentais.. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00302/41299/40472.pdf |
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| Pepin, Jean-francois; Riou, Antoine; Renault, Tristan. |
| Herpes and herpes-like virus infections have been reported in various marine mollusc species associated with high mortality rates. Following the characterisation and genome sequencing of ostreid herpesvirus 1 (OsHV-1), specific diagnostic tools have been developed based on conventional PCR techniques or in situ hybridisation. We have now developed a real-time PCR assay for rapid, sensitive and quantitative detection of OsHV-1, and compared it with a conventional PCR technique described previously. The new assay utilised SYBR® Green chemistry with specific primers C9/C10 targeting the C region. The melt curve analysis of OsHV-1 DNA or DNA extracted from infected material showed only one melting temperature peak (75.75 ± 0.1 °C). The assay had a detection... |
| Tipo: Text |
Palavras-chave: SYBR® Green; Real time PCR; Crassostrea gigas; Ostreid herpesvirus 1; Herpesvirus. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-3951.pdf |
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| Pepin, Jean-francois; Riou, Antoine; Renault, Tristan. |
| Herpes and herpes-like virus infections have been reported in various marine mollusc species associated with high mortality rates. Following the characterisation and genome sequencing of ostreid herpesvirus 1 (OsHV-1), specific diagnostic tools have been developed based on conventional PCR techniques or in situ hybridisation. We have now developed a real-time PCR assay for rapid, sensitive and quantitative detection of OsHV-1, and compared it with a conventional PCR technique described previously. The new assay utilised SYBR® Green chemistry with specific primers C9/C10 targeting the C region, which is present in two copies in the OsHV-1 genome and encodes two proteins of unknown function. The assay was applied successfully to rapid diagnosis (100 minutes)... |
| Tipo: Text |
Palavras-chave: Crassostrea gigas; PCR; Oyster; OsHV 1; Detection; Herpes virus 1. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/acte-3653.pdf |
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| Coeurdacier, Jean-luc; Breuil, Gilles; Pepin, Jean-francois; Mouchel, Olivier; Fauvel, Christian; Laporte, Florent; Thiery, Richard; Castric, J; Raymond, Jean-christophe; Baudin Laurencin, Félix. |
| Ce rapport clôture une collaboration, entreprise courant 97, entre l’Agence Nationale pour la Valorisation de la Recherche (ANVAR), l’Agence Française de Sécurité Sanitaire des Aliments(AFSSA), le Syndicat National des Aquaculteurs Marins (SFAM) et l’Institut Français de Recherche pour l’Exploitation de la Mer (IFREMER). Deux lignées de nodavirus infectant le bar ont été mises en évidence avec une pathogénie variable selon l'âge et les facteurs extérieurs. Ce virus s’est montré d’une résistance exceptionnelle aux facteurs tant physiques que chimiques. Les diagnostics existants ont été adaptés ou réabordés sous un angle différent. Une étude épidémiologique a été réalisée chez les producteurs. La vaccination par peptides synthétiques a permis de sélectionner... |
| Tipo: Text |
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| Ano: 2002 |
URL: http://archimer.ifremer.fr/doc/00167/27874/26120.pdf |
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| Pepin, Jean-francois; Solliec, Gaelle; Vigneron, Vassilia; Thebault, Anne; Lamothe, Julien; Renault, Tristan. |
| The presence of a herpes-type virus has been demonstrated in various species of marine shellfish (bivalves and gastropoda) throughout different regions of the world (Europe, North and South America, Asia, Australia), and is frequently associated with high mortality levels. Nevertheless, apart from the sanitary monitoring in mollusc farming, very little data is available about the detection of this type of virus in the natural populations of bivalves and about the persistence of such viruses in sea water. Various diagnostic tools have been developed in order to detect the oyster herpes virus (Ostreid herpesvirus, OsHV). The presence of this virus has been looked for by PCR in marine water samples coming from fattening ponds, in order to precise the natural... |
| Tipo: Text |
Palavras-chave: Levures; Eau de mer; Diagnostic; Huîtres; Mollusques; OsHV; Herpèsvirus. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/acte-3716.pdf |
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| Breuil, Gilles; Mouchel, Olivier; Fauvel, Christian; Pepin, Jean-francois. |
| Reproduction of nodavirus disease was performed by experimental infection of sea bass eggs during fertilization or at larval stage 4 with 2 genetically distinguishable nodavirus strains (Sb1 and Sb2) isolated from sea bass collected along the Atlantic and Mediterranean French coast. The pathogenicity of the virus strains was assigned after detection of the virus by ELISA and immunohistochemistry (IHC). The Atlantic (Sb1) strain was more pathogenic than the Mediterranean (Sb2) strain during the fertilization step whilst both strains were pathogenic following experimental exposure of 4 d old larvae. Virus lesions developed in the brain 4 to 6 d following experimental exposure. Experimental ELISA proved very sensitive for detecting the nodavirus in Sb1 or Sb2... |
| Tipo: Text |
Palavras-chave: ELISA; Nodavirus; Fish encephalopathy. |
| Ano: 2001 |
URL: http://archimer.ifremer.fr/doc/2001/publication-814.pdf |
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| Renault, Tristan; Allain, Gwenhael; Arzul, Isabelle; Chollet, Bruno; Cobret, Laetitia; De Decker, Sophie; Faury, Nicole; Ferrand, Sylvie; Francois, Cyrille; Garcia, Celine; Haffner, Philippe; Joly, Jean-pierre; Miossec, Laurence; Morga, Benjamin; Nicolas, Jean-louis; Omnes, Emmanuelle; Pepin, Jean-francois; Saulnier, Denis; Schikorski, David; Segarra, Amelie. |
| Abnormal mortality outbreaks occurred in the majority of Pacific oyster (Crassostrea gigas) producing areas in France in 2008 during the summer period. These outbreaks were usually sudden and severe (up to 100%) and affected essentially spat (oysters less than 1 year-old) and juveniles (12 to 18 month-old oysters). Crassostrea gigas was the single affected species Mortality outbreaks were first observed from mid May to mid June in Brittany, Vendée, Charente Maritime and Mediterranean coast A second episode of massive mortality events was reported at the end of June and the beginning of July. All oyster production sites were affected except some particular locations including Arcachon Bay and Brittany. Mortality occurred latter in Arcachon Bay in August.... |
| Tipo: Text |
Palavras-chave: Abnormal mortality; Oysters; Crassostrea gigas; Pathogens. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/00014/12554/9420.pdf |
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| Segarra, Amelie; Faury, Nicole; Pepin, Jean-francois; Renault, Tristan. |
| Massive mortality outbreaks have been reported in France since 2008 among Pacific oysters, Crassostrea gigas, with the detection of a particular OsHV-1 variant called μVar. Virus infection can be induced in healthy spat in experimental conditions allowing to better understand the disease process, including viral gene expression. Although gene expression of other herpesviruses has been widely studied, we provide the first study following viral gene expression of OsHV-1 over time. In this context, an in vivo transcriptomic study targeting 39 OsHV-1 genes was carried out during an experimental infection of Pacific oyster spat. For the first time, several OsHV-1 mRNAs were detected by real-time PCR at 0 h, 2 h, 4 h, 18 h, 26 h and 42 h post injection. Several... |
| Tipo: Text |
Palavras-chave: Crassostrea gigas; OsHV-1; Viral gene expression; Inhibitors of apoptosis; Real time PCR; Elongation factor. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00184/29549/27875.pdf |
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| Registros recuperados: 57 | |
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